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c 12071  (PromoCell)


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    PromoCell c 12071
    C 12071, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+12071/HFDPC-c+Human+Follicle+Dermal+Papilla+Cells/pm41943039-56-14-15
    Average 95 stars, based on 132 article reviews
    c 12071 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Mixture of Mastic Gum and Peppermint Extracts Promotes Hair Growth and Health in Vitro and in C57BL/6 Mice.
    Article Snippet: .. Human follicle dermal papilla cells (HFDPCs) were purchased from PromoCell (C-12071; Heidelberg, Germany) and cultured in ready-to-use follicle dermal papilla cell growth medium (C26501; PromoCell) supplemented with growth medium supplement mix (C-39625; PromoCell). .. RAW 264.7 cells were purchased from American Type Culture Collection (TIB-71; Manassas, VA, United States) and cultured in Dulbecco’s modified Eagle’s serum (12-604Q; Lonza, Basel, Switzerland) supplemented with 100 mg/mL fetal bovine serum (16000-044; Gibco, Grand Island, New York, MT, United States) and 100 U/ mL penicillin-streptavidin (15140122; Gibco).

    Article Title: Age-related downregulation of dihydrotestosterone-inactivating enzymes in human scalp sebaceous glands
    Article Snippet: SEB-1 male-derived immortalized sebocyte cells were kindly provided by the Penn State Research Foundation and cultured in Reduced Serum DMEM/Ham's F-12 Medium (Nacalai Tesque) supplemented with 5% fetal bovine serum, 180 μM adenine, 0.4 μg/ml hydrocortisone, 10 ng/ml epidermal growth factor, and 10 μM isoproterenol. .. Human DPCs, derived from the temple region of a 53-year-old Caucasian male, were obtained (c-12071, PromoCell) and cultured in Follicle Dermal Papilla Cell Growth Medium (Ready-to-use) (c-26501, PromoCell). ..

    Article Title: Mixture of Mastic Gum and Peppermint Extracts Promotes Hair Growth and Health in Vitro and in C57BL/6 Mice
    Article Snippet: .. Human follicle dermal papilla cells (HFDPCs) were purchased from PromoCell (C-12071; Heidelberg, Germany) and cultured in ready-to-use follicle dermal papilla cell growth medium (C-26501; PromoCell) supplemented with growth medium supplement mix (C-39625; PromoCell). .. RAW 264.7 cells were purchased from American Type Culture Collection (TIB-71; Manassas, VA, United States) and cultured in Dulbecco’s modified Eagle’s serum (12-604Q; Lonza, Basel, Switzerland) supplemented with 100 mg/mL fetal bovine serum (16000-044; Gibco, Grand Island, New York, MT, United States) and 100 U/mL penicillin-streptavidin (15140122; Gibco).

    Article Title: Age-related downregulation of dihydrotestosterone-inactivating enzymes in human scalp sebaceous glands.
    Article Snippet: SEB-1 male-derived immortalized sebocyte cells were kindly provided by the Penn State Research Foundation and cultured in Reduced Serum DMEM/Ham's F-12 Medium (Nacalai Tesque) supplemented with 5% fetal bovine serum, 180 μM adenine, 0.4 μg/ml hydrocortisone, 10 ng/ml epidermal growth factor, and 10 μM isoproterenol. .. Human DPCs, derived from the temple region of a 53-year-old Caucasian male, were obtained (c-12071, PromoCell) and cultured in Follicle Dermal Papilla Cell Growth Medium (Ready-to-use) (c-26501, PromoCell). ..

    Derivative Assay:

    Article Title: Age-related downregulation of dihydrotestosterone-inactivating enzymes in human scalp sebaceous glands
    Article Snippet: SEB-1 male-derived immortalized sebocyte cells were kindly provided by the Penn State Research Foundation and cultured in Reduced Serum DMEM/Ham's F-12 Medium (Nacalai Tesque) supplemented with 5% fetal bovine serum, 180 μM adenine, 0.4 μg/ml hydrocortisone, 10 ng/ml epidermal growth factor, and 10 μM isoproterenol. .. Human DPCs, derived from the temple region of a 53-year-old Caucasian male, were obtained (c-12071, PromoCell) and cultured in Follicle Dermal Papilla Cell Growth Medium (Ready-to-use) (c-26501, PromoCell). ..

    Article Title: Age-related downregulation of dihydrotestosterone-inactivating enzymes in human scalp sebaceous glands.
    Article Snippet: SEB-1 male-derived immortalized sebocyte cells were kindly provided by the Penn State Research Foundation and cultured in Reduced Serum DMEM/Ham's F-12 Medium (Nacalai Tesque) supplemented with 5% fetal bovine serum, 180 μM adenine, 0.4 μg/ml hydrocortisone, 10 ng/ml epidermal growth factor, and 10 μM isoproterenol. .. Human DPCs, derived from the temple region of a 53-year-old Caucasian male, were obtained (c-12071, PromoCell) and cultured in Follicle Dermal Papilla Cell Growth Medium (Ready-to-use) (c-26501, PromoCell). ..



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    Effect of SCFF on HFDPC and HaCaT cell viability and proliferation. <t>HFDPCs</t> and HaCaT cells were treated with different concentrations of SCFF as indicated for 24 h. ( a ) HFDPC and ( b ) HaCaT viability and proliferation were assessed at various SCFF concentrations (50, 100, 250, 500, 1000, 2500, 5000 μg/mL) using a CCK-8 assay. Values are mean ± SD of three independent experiments. ( a – c ) *** p < 0.001, ** p < 0.01 and * p < 0.05 versus NC group (100%). ( c ) Representative images of cellular proliferation activity in HFDPCs and HaCaT cells after 48 h of incubation ( n = 3; Scale bar = 200 μm). SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, <t>human</t> <t>follicle</t> dermal papilla cells; NC, negative control.
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    Image Search Results


    Effect of SCFF on HFDPC and HaCaT cell viability and proliferation. HFDPCs and HaCaT cells were treated with different concentrations of SCFF as indicated for 24 h. ( a ) HFDPC and ( b ) HaCaT viability and proliferation were assessed at various SCFF concentrations (50, 100, 250, 500, 1000, 2500, 5000 μg/mL) using a CCK-8 assay. Values are mean ± SD of three independent experiments. ( a – c ) *** p < 0.001, ** p < 0.01 and * p < 0.05 versus NC group (100%). ( c ) Representative images of cellular proliferation activity in HFDPCs and HaCaT cells after 48 h of incubation ( n = 3; Scale bar = 200 μm). SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells; NC, negative control.

    Journal: Microorganisms

    Article Title: Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells

    doi: 10.3390/microorganisms14040929

    Figure Lengend Snippet: Effect of SCFF on HFDPC and HaCaT cell viability and proliferation. HFDPCs and HaCaT cells were treated with different concentrations of SCFF as indicated for 24 h. ( a ) HFDPC and ( b ) HaCaT viability and proliferation were assessed at various SCFF concentrations (50, 100, 250, 500, 1000, 2500, 5000 μg/mL) using a CCK-8 assay. Values are mean ± SD of three independent experiments. ( a – c ) *** p < 0.001, ** p < 0.01 and * p < 0.05 versus NC group (100%). ( c ) Representative images of cellular proliferation activity in HFDPCs and HaCaT cells after 48 h of incubation ( n = 3; Scale bar = 200 μm). SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells; NC, negative control.

    Article Snippet: HFDPCs (C-12071; Promocell, Heidelberg, Germany) were cultured in Follicle Dermal Papilla Cell Growth Medium (C-26501; Promocell) supplemented with 1% antibiotic–antimycotic (Gibco; Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: CCK-8 Assay, Activity Assay, Incubation, Negative Control

    Hair growth-related and AGA-inducing signature gene expression following SCFF treatment in HFDPCs. HFDPCs were treated with different SCFF concentrations for 24 h, and gene expression was evaluated using qPCR. ( a ) Hair growth-related factors (KGF, IGF-1, and HGF). ( b ) AGA-inducing factors (AR and TGF-β2). MXD at 5 μg/mL was used as PC for TGF-β2, and 2.5 μg/mL MXD was used as PC for other markers. Data are mean ± SD of three independent experiments. *** p < 0.001, ** p < 0.01 and * p < 0.05 versus NC group (100%). SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells; MXD, minoxidil; PC, positive control.

    Journal: Microorganisms

    Article Title: Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells

    doi: 10.3390/microorganisms14040929

    Figure Lengend Snippet: Hair growth-related and AGA-inducing signature gene expression following SCFF treatment in HFDPCs. HFDPCs were treated with different SCFF concentrations for 24 h, and gene expression was evaluated using qPCR. ( a ) Hair growth-related factors (KGF, IGF-1, and HGF). ( b ) AGA-inducing factors (AR and TGF-β2). MXD at 5 μg/mL was used as PC for TGF-β2, and 2.5 μg/mL MXD was used as PC for other markers. Data are mean ± SD of three independent experiments. *** p < 0.001, ** p < 0.01 and * p < 0.05 versus NC group (100%). SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells; MXD, minoxidil; PC, positive control.

    Article Snippet: HFDPCs (C-12071; Promocell, Heidelberg, Germany) were cultured in Follicle Dermal Papilla Cell Growth Medium (C-26501; Promocell) supplemented with 1% antibiotic–antimycotic (Gibco; Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Gene Expression, Positive Control

    The regulation of cellular senescence by SCFF in HFDPCs. HFDPCs were treated with different concentrations of SCFF for 24 h, followed by analysis using qPCR and SA-β-gal staining. ( a , b ) Anti-aging factors (SIRT1, SIRT7, COL13A1, and p21). ( c ) SA-β-gal staining in young (P6) and senescent (P16) passage cells. Quantitative and representative images of SA-β-gal-positive cells compared with senescent cells (Scale bar = 200 μm). For SIRT, 1 μg/mL fisetin (Fis) was used as a positive control (PC), and for COL13 and p21, 1 μg/mL Fis was used as PC. Data are the mean ± SD of three independent experiments. **** p < 0.0001, *** p < 0.001, ** p < 0.01, and * p < 0.05 versus the negative control (NC) and the senescent group (100%). SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells.

    Journal: Microorganisms

    Article Title: Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells

    doi: 10.3390/microorganisms14040929

    Figure Lengend Snippet: The regulation of cellular senescence by SCFF in HFDPCs. HFDPCs were treated with different concentrations of SCFF for 24 h, followed by analysis using qPCR and SA-β-gal staining. ( a , b ) Anti-aging factors (SIRT1, SIRT7, COL13A1, and p21). ( c ) SA-β-gal staining in young (P6) and senescent (P16) passage cells. Quantitative and representative images of SA-β-gal-positive cells compared with senescent cells (Scale bar = 200 μm). For SIRT, 1 μg/mL fisetin (Fis) was used as a positive control (PC), and for COL13 and p21, 1 μg/mL Fis was used as PC. Data are the mean ± SD of three independent experiments. **** p < 0.0001, *** p < 0.001, ** p < 0.01, and * p < 0.05 versus the negative control (NC) and the senescent group (100%). SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells.

    Article Snippet: HFDPCs (C-12071; Promocell, Heidelberg, Germany) were cultured in Follicle Dermal Papilla Cell Growth Medium (C-26501; Promocell) supplemented with 1% antibiotic–antimycotic (Gibco; Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Staining, Positive Control, Negative Control

    Overall effect of SCFF in HFDPCs and HaCaT cells. In keratinocytes, SCFF enhanced barrier function and antioxidant activity. SCFF not only regulated cellular aging through longevity-, collagen-, and p21-related genes, but also promoted hair growth by increasing the expression of hair-growth–associated factors and suppressing hair loss-inducing factors in HFDPCs. SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells.

    Journal: Microorganisms

    Article Title: Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells

    doi: 10.3390/microorganisms14040929

    Figure Lengend Snippet: Overall effect of SCFF in HFDPCs and HaCaT cells. In keratinocytes, SCFF enhanced barrier function and antioxidant activity. SCFF not only regulated cellular aging through longevity-, collagen-, and p21-related genes, but also promoted hair growth by increasing the expression of hair-growth–associated factors and suppressing hair loss-inducing factors in HFDPCs. SCFF, Staphylococcus capitis KMH304 ferment filtrate; HFDPCs, human follicle dermal papilla cells.

    Article Snippet: HFDPCs (C-12071; Promocell, Heidelberg, Germany) were cultured in Follicle Dermal Papilla Cell Growth Medium (C-26501; Promocell) supplemented with 1% antibiotic–antimycotic (Gibco; Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Antioxidant Activity Assay, Expressing